Microwave Techniques and ProtocolsGiberson, Richard T.; Demaree Jr., Richard S. (Eds.)
2001, 230 p. 183 illus., Hardcover
ISBN: 978-0-89603-903-2
About this book
Richard Giberson and Richard Demaree, Jr, have collected a wide range of time-saving microwave techniques for processing biological samples for evaluation by many different microscopic methods. Described in step-by-step detail by hands-on researchers, these readily reproducible protocols include both optimized classic methods and such state-of-the art techniques as in vivo labeling, formalin fixation of fresh tissue, vacuum processing, and processing for scanning electron microscopy. Each stand-alone microwave method has been handcrafted by a researcher who regularly uses it to ensure processing success and the brightest quality result.
Written for:
Microscopists and pathologists
Reviews
"...if you study archived pathology sections, rat hippocampal slices, decalcification, sediments, Allium and Drosophilia nuclei, motor endplates or skin, there is something for you here. Highly specialized therefore, but invaluable if you need this procedure." -Ultramicroscopy
"Microwave Techniques and Protocols is both informative, providing a broad perspective on how microwave treatment of samples is being used, and useful as a procedural manual for performing microwave sample treatment and for developing your own techniques." -Journal of Chemical Neuroanatomy
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本帖最后由 drjiachen 于 2008-12-15 10:29 编辑 ]
.................................................................................................. v
Contributors ........................................................................................ ix
1 Overview of Microwave-Assisted Tissue Processing
for Transmission Electron Microscopy
Richard S. Demaree, Jr.
and Richard T. Giberson.......................................1
2 Vacuum-Assisted Microwave Processing
of Animal Tissues for Electron Microscopy
Richard T. Giberson .................................................13
3 Vacuum–Microwave Combination for Processing
Plant Tissues for Electron Microscopy
William A. Russin and Christina L. Trivett ............25
4 Basic Procedure for Electron Microscopy Processing
and Staining in Clinical Laboratory Using
Microwave Oven
Ronald L. Austin .......................................................37
5 Specimen Preparation for Thin-Section Electron
Microscopy Utilizing Microwave-Assisted Rapid
Processing in a Veterinary Diagnostic Laboratory
Robert W. Nordhausen and Bradd C. Barr ............49
6 Microwave Processing of Archived Pathology
Specimens for Ultrastructural Examination
Robert J. Munn and Phillip J. Vogt ........................67
7 Microwave Fixation of Rat Hippocampal Slices
Marcia D. Feinberg, Karen M. Szumowski,
and Kristen M. Harris .........................................75
8 Microwave Processing Techniques for Biological
Samples in a Service Laboratory
Lou Ann Miller .........................................................89
vii
CONTENTS
9 Microwave-Accelerated Decalcification: Useful
Methods for Research and Clinical Laboratories
Victoria J. Madden .................................................101
10 Microwave Processing of Sediment Samples
Dawn Lavoie, Janet Watkins,
and Yoko Furukawa ..........................................123
11 Microwave Polymerization in Thin Layers
of London Resin White Allows Selection
of Specimens for Immunogold Labeling
Jennifer E. Lonsdale, Kent L. McDonald,
and Russell L. Jones .........................................139
12 In Vivo Microwave-Assisted Labeling of Allium
and Drosophila Nuclei
Mark A. Sanders and David M. Gartner...............155
13 Microwave-Assisted Cytochemistry: Accelerated
Visualization of Acetylcholinesterase at Motor
Endplates
John P. Petralia and Kenneth R. Mills .................165
14 Microwave-Assisted Immunoelectron Microscopy
of Skin: Localization of Laminin, Type IV
Collagen, and Bullous Pemphigoid Antigen
John P. Petrali and Kenneth R. Mills ...................173
15 Microwave Paraffin Techniques for Botanical
Tissues
Denise Schichnes, Jeffrey A. Nemson,
and Steven E. Ruzin ..........................................181
16 Microwave-Assisted Formalin Fixation of Fresh
Tissue: A Comparative Study
Richard T. Giberson and Douglas E. Elliott ........191
17 Microwave-Assisted Processing of Biological
Samples for Scanning Electron Microscopy
Richard S. Demaree, Jr. .........................................209
Index ................................................................................................ 217
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谢谢楼主分享..............................................
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